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intracellular m abscessus atcc 19977  (ATCC)


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    ATCC intracellular m abscessus atcc 19977
    Frequency of mutational M. abscessus resistance (FoR) to apramycin (APR) and amikacin (AMK). (a) M. abscessus ATCC 19977. (b) M. abscessus NR-44261. All studies were performed on selective LB agar plates incubated at 37 °C for 5 days. Data are represented as scatter plot, the centre bars represent the geometric mean ( n = 3–4 technical replicates per strain and drug concentration tested). The lower limits of quantification (LoQ) defined by ≤ 1 CFU per plate are indicated by dotted lines. Circular symbols at the LoQ are equivalent to one resistant CFU, triangular symbols pointing downward indicate the absence of resistant CFUs and thus a frequency below that LoQ.
    Intracellular M Abscessus Atcc 19977, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1174 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/intracellular m abscessus atcc 19977/product/ATCC
    Average 99 stars, based on 1174 article reviews
    intracellular m abscessus atcc 19977 - by Bioz Stars, 2026-02
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    1) Product Images from "Multicentre preclinical profiling of apramycin for the treatment of nontuberculous mycobacteria"

    Article Title: Multicentre preclinical profiling of apramycin for the treatment of nontuberculous mycobacteria

    Journal: eBioMedicine

    doi: 10.1016/j.ebiom.2025.106103

    Frequency of mutational M. abscessus resistance (FoR) to apramycin (APR) and amikacin (AMK). (a) M. abscessus ATCC 19977. (b) M. abscessus NR-44261. All studies were performed on selective LB agar plates incubated at 37 °C for 5 days. Data are represented as scatter plot, the centre bars represent the geometric mean ( n = 3–4 technical replicates per strain and drug concentration tested). The lower limits of quantification (LoQ) defined by ≤ 1 CFU per plate are indicated by dotted lines. Circular symbols at the LoQ are equivalent to one resistant CFU, triangular symbols pointing downward indicate the absence of resistant CFUs and thus a frequency below that LoQ.
    Figure Legend Snippet: Frequency of mutational M. abscessus resistance (FoR) to apramycin (APR) and amikacin (AMK). (a) M. abscessus ATCC 19977. (b) M. abscessus NR-44261. All studies were performed on selective LB agar plates incubated at 37 °C for 5 days. Data are represented as scatter plot, the centre bars represent the geometric mean ( n = 3–4 technical replicates per strain and drug concentration tested). The lower limits of quantification (LoQ) defined by ≤ 1 CFU per plate are indicated by dotted lines. Circular symbols at the LoQ are equivalent to one resistant CFU, triangular symbols pointing downward indicate the absence of resistant CFUs and thus a frequency below that LoQ.

    Techniques Used: Incubation, Concentration Assay

    Dose-dependent killing of planktonic and intracellular NTMs by apramycin (APR) in comparison to amikacin (AMK). (a) Time- and dose-dependent CFU reduction of M. abscessus reference strains ATCC 19977 (smooth) and NR-44261 (rough) in CAMHB (pH 7.4) determined by sampling, plating, and CFU counting at different time points over 120 h. (b) Luminescence based killing kinetics in standard Middlebrook 7H9 (pH 6.7) and in a 7H9 medium that has been buffered with MES to pH 6.0. (c) Dose–response kill curve for stationary-phase M. abscessus ATCC 19977 in caseum surrogate following five days of drug exposure (mean ± SD; n = 3 technical replicates). (d) Dose response curve of intracellular killing of M. abscessus ATCC 19977 by day 3 and of M. avium ATCC 19698 by day 7 (mean ± SD; n = 3 biological replicates). MXF, moxifloxacin; CLA, clarithromycin.
    Figure Legend Snippet: Dose-dependent killing of planktonic and intracellular NTMs by apramycin (APR) in comparison to amikacin (AMK). (a) Time- and dose-dependent CFU reduction of M. abscessus reference strains ATCC 19977 (smooth) and NR-44261 (rough) in CAMHB (pH 7.4) determined by sampling, plating, and CFU counting at different time points over 120 h. (b) Luminescence based killing kinetics in standard Middlebrook 7H9 (pH 6.7) and in a 7H9 medium that has been buffered with MES to pH 6.0. (c) Dose–response kill curve for stationary-phase M. abscessus ATCC 19977 in caseum surrogate following five days of drug exposure (mean ± SD; n = 3 technical replicates). (d) Dose response curve of intracellular killing of M. abscessus ATCC 19977 by day 3 and of M. avium ATCC 19698 by day 7 (mean ± SD; n = 3 biological replicates). MXF, moxifloxacin; CLA, clarithromycin.

    Techniques Used: Comparison, Sampling



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    Frequency of mutational M. abscessus resistance (FoR) to apramycin (APR) and amikacin (AMK). (a) M. abscessus ATCC 19977. (b) M. abscessus NR-44261. All studies were performed on selective LB agar plates incubated at 37 °C for 5 days. Data are represented as scatter plot, the centre bars represent the geometric mean ( n = 3–4 technical replicates per strain and drug concentration tested). The lower limits of quantification (LoQ) defined by ≤ 1 CFU per plate are indicated by dotted lines. Circular symbols at the LoQ are equivalent to one resistant CFU, triangular symbols pointing downward indicate the absence of resistant CFUs and thus a frequency below that LoQ.
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    Frequency of mutational M. abscessus resistance (FoR) to apramycin (APR) and amikacin (AMK). (a) M. abscessus ATCC 19977. (b) M. abscessus NR-44261. All studies were performed on selective LB agar plates incubated at 37 °C for 5 days. Data are represented as scatter plot, the centre bars represent the geometric mean ( n = 3–4 technical replicates per strain and drug concentration tested). The lower limits of quantification (LoQ) defined by ≤ 1 CFU per plate are indicated by dotted lines. Circular symbols at the LoQ are equivalent to one resistant CFU, triangular symbols pointing downward indicate the absence of resistant CFUs and thus a frequency below that LoQ.
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    Image Search Results


    Frequency of mutational M. abscessus resistance (FoR) to apramycin (APR) and amikacin (AMK). (a) M. abscessus ATCC 19977. (b) M. abscessus NR-44261. All studies were performed on selective LB agar plates incubated at 37 °C for 5 days. Data are represented as scatter plot, the centre bars represent the geometric mean ( n = 3–4 technical replicates per strain and drug concentration tested). The lower limits of quantification (LoQ) defined by ≤ 1 CFU per plate are indicated by dotted lines. Circular symbols at the LoQ are equivalent to one resistant CFU, triangular symbols pointing downward indicate the absence of resistant CFUs and thus a frequency below that LoQ.

    Journal: eBioMedicine

    Article Title: Multicentre preclinical profiling of apramycin for the treatment of nontuberculous mycobacteria

    doi: 10.1016/j.ebiom.2025.106103

    Figure Lengend Snippet: Frequency of mutational M. abscessus resistance (FoR) to apramycin (APR) and amikacin (AMK). (a) M. abscessus ATCC 19977. (b) M. abscessus NR-44261. All studies were performed on selective LB agar plates incubated at 37 °C for 5 days. Data are represented as scatter plot, the centre bars represent the geometric mean ( n = 3–4 technical replicates per strain and drug concentration tested). The lower limits of quantification (LoQ) defined by ≤ 1 CFU per plate are indicated by dotted lines. Circular symbols at the LoQ are equivalent to one resistant CFU, triangular symbols pointing downward indicate the absence of resistant CFUs and thus a frequency below that LoQ.

    Article Snippet: APR was found to kill intracellular M. abscessus ATCC 19977 (E max = −0.67 log CFU/mL) and intracellular M. avium ATCC 19698 (E max = −1.45 log CFU/mL) in a dose-dependent manner, with the M. avium reference strain (MIC = 4 μg/mL at 37 °C) appearing to be more susceptible to intracellular APR killing than the M. abscessus reference strain (MIC = 0.5 μg/mL at 37 °C) ( d).

    Techniques: Incubation, Concentration Assay

    Dose-dependent killing of planktonic and intracellular NTMs by apramycin (APR) in comparison to amikacin (AMK). (a) Time- and dose-dependent CFU reduction of M. abscessus reference strains ATCC 19977 (smooth) and NR-44261 (rough) in CAMHB (pH 7.4) determined by sampling, plating, and CFU counting at different time points over 120 h. (b) Luminescence based killing kinetics in standard Middlebrook 7H9 (pH 6.7) and in a 7H9 medium that has been buffered with MES to pH 6.0. (c) Dose–response kill curve for stationary-phase M. abscessus ATCC 19977 in caseum surrogate following five days of drug exposure (mean ± SD; n = 3 technical replicates). (d) Dose response curve of intracellular killing of M. abscessus ATCC 19977 by day 3 and of M. avium ATCC 19698 by day 7 (mean ± SD; n = 3 biological replicates). MXF, moxifloxacin; CLA, clarithromycin.

    Journal: eBioMedicine

    Article Title: Multicentre preclinical profiling of apramycin for the treatment of nontuberculous mycobacteria

    doi: 10.1016/j.ebiom.2025.106103

    Figure Lengend Snippet: Dose-dependent killing of planktonic and intracellular NTMs by apramycin (APR) in comparison to amikacin (AMK). (a) Time- and dose-dependent CFU reduction of M. abscessus reference strains ATCC 19977 (smooth) and NR-44261 (rough) in CAMHB (pH 7.4) determined by sampling, plating, and CFU counting at different time points over 120 h. (b) Luminescence based killing kinetics in standard Middlebrook 7H9 (pH 6.7) and in a 7H9 medium that has been buffered with MES to pH 6.0. (c) Dose–response kill curve for stationary-phase M. abscessus ATCC 19977 in caseum surrogate following five days of drug exposure (mean ± SD; n = 3 technical replicates). (d) Dose response curve of intracellular killing of M. abscessus ATCC 19977 by day 3 and of M. avium ATCC 19698 by day 7 (mean ± SD; n = 3 biological replicates). MXF, moxifloxacin; CLA, clarithromycin.

    Article Snippet: APR was found to kill intracellular M. abscessus ATCC 19977 (E max = −0.67 log CFU/mL) and intracellular M. avium ATCC 19698 (E max = −1.45 log CFU/mL) in a dose-dependent manner, with the M. avium reference strain (MIC = 4 μg/mL at 37 °C) appearing to be more susceptible to intracellular APR killing than the M. abscessus reference strain (MIC = 0.5 μg/mL at 37 °C) ( d).

    Techniques: Comparison, Sampling